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. 2011 Mar 29;152(6):2247–2255. doi: 10.1210/en.2010-1036

Fig. 1.

Fig. 1.

IRE1α-XBP1 pathway was activated in the liver of mice with insulin resistance and hyperinsulinemia induced by the HFD. C57BL/6 (B6) mice (8–10 wk) were fed with ND (n = 8) or HFD (n = 7) for 8 wk. A, Plasma insulin level was evaluated after an overnight fast. Basal Akt activation (B) and TG content (C) in the liver were measured. D, IRE1α phosphorylation at serine 724 in the liver was detected by immunoblotting with the indicated antibodies. E, The spliced form of XBP1 mRNA in the liver was evaluated by RT-PCR. The transcript 36B4 was used as a loading control. F, Nuclear XBP1 (nXBP1) in the liver was estimated with immunoblotting with the anti-XBP1 antibody and normalized to a housekeeping nuclear protein PARP1. Data represent mean ± sem.