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. 2011 Apr 12;152(6):2364–2376. doi: 10.1210/en.2010-1435

Table 1.

Primers used for conventional PCR and real-time PCR

Conventional PCR primers
Gene name Accession no. Sense primer Position Antisense primer Position
SynCAM1 promoter NT_033899 tatttttattagagacggagtttc 18,939,510-487 gcggacagctaatgagatg 18,937,656-578
DN SynCAM1 AF539424 agatctaggcgtgtacggtgggaggtcta 122–145 gtcgacgcaaaatagcggcccagaatgat 1405–1428
DN SynCAM1 genotyping AF539424 and U55762 cctcccacaacaaccaccaccact 1140–1163 gccctcgccggacacgctgaac 759–780
CASK NM_022184 tattcggtcgaccggaccatggccgacgacgacgtgct 25–50 tattcgccgcgggcaatagacccaggagaccgggac 2737–2760
Real-time PCR primers and probe
Gene name Accession no. Sense primer Sense primer position Antisense primer Antisense primer position Probe Probe position
SynCAM1 AF539424 ccctcctcccacaacaacc 1033-1051 ggtcccctcttcacctgctc 1107–1126 cacctccatccttaccatcatcacagattct 1075–1105