Table 1.
Conventional PCR primers | |||||
---|---|---|---|---|---|
Gene name | Accession no. | Sense primer | Position | Antisense primer | Position |
SynCAM1 promoter | NT_033899 | tatttttattagagacggagtttc | 18,939,510-487 | gcggacagctaatgagatg | 18,937,656-578 |
DN SynCAM1 | AF539424 | agatctaggcgtgtacggtgggaggtcta | 122–145 | gtcgacgcaaaatagcggcccagaatgat | 1405–1428 |
DN SynCAM1 genotyping | AF539424 and U55762 | cctcccacaacaaccaccaccact | 1140–1163 | gccctcgccggacacgctgaac | 759–780 |
CASK | NM_022184 | tattcggtcgaccggaccatggccgacgacgacgtgct | 25–50 | tattcgccgcgggcaatagacccaggagaccgggac | 2737–2760 |
Real-time PCR primers and probe | |||||||
---|---|---|---|---|---|---|---|
Gene name | Accession no. | Sense primer | Sense primer position | Antisense primer | Antisense primer position | Probe | Probe position |
SynCAM1 | AF539424 | ccctcctcccacaacaacc | 1033-1051 | ggtcccctcttcacctgctc | 1107–1126 | cacctccatccttaccatcatcacagattct | 1075–1105 |