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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: J Immunol. 2011 Mar 23;186(9):5489–5496. doi: 10.4049/jimmunol.1001623

Figure 2.

Figure 2

LPS-induced IL-1β mRNA splicing requires MyD88 and TIRAP. Purified human (A) or mouse (B) platelets were treated with or without LPS, soluble CD14, and LPS-binding protein and for 3 hours at 37°C. RNA was isolated from cell lysates and spliced IL-1β mRNA was measured by real-time quantitative PCR. Peptide-based decoy peptide inhibitors were used at a concentration of 50 μM. IRAK inhibitor was used at a concentration of 2 μM Error bars = +/− SE, N=7 human donors (A). Error bar = +/− 1 SE, N=5 experiments of pooled mice blood from 2–4 mice each (B).