LPS activates JNK and Akt in human platelets. Platelets were treated with LPS, soluble CD14, and LPS-binding protein before lysates were made at the stated times. A significant increase in JNK (A) and Akt phosphorylation, (B) pTyr308-Akt and (C) pSer473-Akt, was measured by ELISA. Data is expressed as the ratio of phospho-proteins to total kinase protein. Error bars = +/− 1 SE, N=3.