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. 2011 May 16;2:280–291. doi: 10.7150/jca.2.280

Figure 1.

Figure 1

Generation of immunoadhesin HN125. A. Diagram of expression plasmid pMH142 for HN125 expression in human HEK-293F cells as NH2-terminal fusions to human IgG1 Fc (hFc) (Hinge-CH2-CH3). CMV IE Enhancer/Promoter: human cytomegalovirus immediate-early enhancer/promoter; IL2 Sig: IL2 signal peptide; Tbgh: poly A bovine growth hormone tail; ori: origin of replication; Kan.: kanamycin resistance. B. The structural model of HN125. IAB is the functional binding domain in mesothelin for MUC16. CH2 and CH3: second and third constant domains of a human IgG1, respectively. C. SDS-PAGE analysis of HN125. Molecular weights: non-reduced dimers (~75 kDa) and reduced monomers (~37 kDa).