Figure 6.

HN125-mediated killing of CA125-expressing cancer cells via ADCC. A. OVCAR3 cells were incubated with PBMC and either 10 μg/mL of HN125 or a control human IgG. Percent of cell death was measured using a LDH kit. About 35% of target cells were killed when treated with HN125 (p values < 0.05). B and C. NK cells isolated from peripheral blood of 2 healthy donors. OVCAR3 (B) and ECC-1 (C) cells used as targets were loaded with ⁵¹Cr. Cell lysis was determined by the release of ⁵¹Cr. Lysis assays were conducted in the presence or absence of HN125 as designated. Representative data shown is for effector:target ratio of 12:1. Each E:T was tested in triplicate in three separate experiments.