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. 2011 Mar 15;117(18):4736–4745. doi: 10.1182/blood-2010-10-311845

Figure 3.

Figure 3

CD23.CAR+ T lymphocytes lack cytotoxic activity against normal B lymphocytes. (A) MFI of CD23 antigen on B cells isolated from the peripheral blood of healthy donors and CLL patients is shown. Percentages of double-positive CD19/CD23 cells were comparable in healthy donors and CLL patients at levels ≥ 90% (data not shown), as expected. **P ≤ .005 comparing healthy donors and CLL-derived B cells. (B) Cytotoxic activity of control NT, CD19.CAR+, and CD23.CAR+ T lymphocytes obtained from healthy donors against purified CD20+ B cells obtained from the peripheral blood of healthy donors. Cytotoxic activity was evaluated in a 51Cr-release assay, and results are shown at E:T ratios of 40:1, 20:1, 10:1, and 5:1. Data represent the means ± SD for 3 different T-cell lines. *P ≤ .05 comparing CAR+ with NT T lymphocytes. (C) Cytotoxic activity of control NT, CD19.CAR+, and CD23.CAR+ T lymphocytes obtained from CLL patients against purified CD20+ B cells labeled with CFSE. Cytotoxic activity was evaluated in a 24-hour coculture experiment, and results are shown at an E:T ratio of 3:1. The experiment shown is representative of 3 different experiments.