Skip to main content
. 2011 Jun 15;138(12):2511–2521. doi: 10.1242/dev.062505

Fig. 4.

Fig. 4.

Disruption of the first wave of spermatogenesis in RanBPM−/− mice. (A-D) Wild-type (WT; A,C) and RanBPM−/− (B,D) 3-week-old testis sections stained with Hematoxylin and Eosin (H&E; A,B) or processed for TUNEL assay (C,D) show a lack of spermatids and a high level of germ cell apoptosis (brown cells indicated by black arrows) in the mutant (compare C and D). B′ and D′ are high magnification images of boxed areas in B and D, respectively. Sg, spermatogonia; Sc, spermatocyte; Sd, spermatid. Scale bars: 100 μm. (E) RT-PCR analysis for stage-specific markers of spermatogenesis on 3-week-old WT and knockout testes. GAPDH and β-Actin were used as standards. (F) Analysis of meiotic progression of spermatocytes. Immunofluorescence labeling of spermatocyte spreads at pachytene (top panels) and diplotene (bottom panels) from 3-week-old WT and RanBPM−/− mice. The synaptonemal complex (SC) was labeled with anti-Scp3 antibodies (red). The sex body was visualized using anti-γH2AX antibodies (green) and DNA using DAPI (blue).