Skip to main content
. 2011 Jun 15;138(12):2511–2521. doi: 10.1242/dev.062505

Fig. 5.

Fig. 5.

Developmental expression of RanBPM in testis. (A,B) β-Galactosidase (β-gal) staining of heterozygous mice testes showing RanBPM expression at embryonic stage (E)17.5 (A) and its colocalization with the germ cell marker MVH (B). (C-E) β-Gal staining of RanBPM+/− testes at birth (C), postnatal day (P)5 (D) and 2 weeks (E). Staining duration was identical for sections C to E. Counterstaining was performed using Neutral Red. (F-G) β-Gal staining of adult RanBPM+/− testis sections (F) and whole-mount seminiferous tubules (G), showing a high and dynamic expression of RanBPM at different stages of the seminiferous tubule cycle. F′ and F″ are high magnification images of insets in F showing a high level of expression in the germinal cell lineage. Note the high level of expression in spermatocytes (Sc in F′) and Sertoli cells (white arrowheads). lu, lumen; lc, Leydig cells; Sd, spermatids. Scale bars: 400 μm in A; 100 μm in C-F; 2 mm in G. (H-J) RanBPM protein is expressed and upregulated during testis maturation. (H) Western blot analysis of whole testis lysates from P1 to 4 months using an anti-RanBPM antibody. β-Actin was used as loading control. (I-J) RanBPM protein is detected by immunohistochemistry in spermatocytes from 2.5-week-old wild-type (WT; I) testis sections but not in age-matched mutant sections (J). Scale bar: 100 μm.