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. 2011 Jun 15;138(12):2511–2521. doi: 10.1242/dev.062505

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Fig. 6. — Cell-autonomous role of RanBPM in germ cells. (A,A′,C,C′,E,E′) β-Galactosidase (β-gal) staining of 23-day-old (P23) testis sections from heterozygous (+/−; A,A′) animals or chimeras generated by blastocyst injection of heterozygous (+/− ES cells; C,C′) or mutant (−/− ES cells; E,E′) embryonic stem cells shows a total lack of β-gal+ germ cells in the tubules of chimeras generated with the −/− ES cells (E,E′). Note the abundant staining in the supporting cells surrounding the tubules in E and E′. (G,H) β-Gal staining of 10-day-old chimeras generated by either +/− (G) or −/− (H) ES cells showing no difference in the contribution of the ES cells to the tubules of either type of chimeras (H). (B,D,F) Corresponding Hematoxylin and Eosin (H&E) staining of P23 testis sections from heterozygous animal (B) or chimeras generated from +/− (D) or −/− (F) ES cell injection. Scale bars: 100 μm.