Fig. 8.

Perinatal loss of oocytes in RanBPM^−/− mice. (A,B) γH2AX-immunostained ovary cryosections showing comparable numbers of meiotic oocytes between wild-type (WT; A) and RanBPM^−/− (B) embryonic day (E)17.5 animals. (C,D) Immunolabeling of germ cells using anti-MVH (red) and anti-γH2AX (green) antibodies on paraffin ovary sections from postnatal day (P)0 WT (C) and RanBPM^−/− (D) mice. Note the oocyte depletion at this stage in the mutant ovaries. (E,F) Immunolabeling for MVH on paraffin ovary sections from P5 WT (E) and RanBPM^−/− (F) mice. Sections counterstained with DAPI (blue). (G,H) β-Gal staining of a P2 heterozygous ovary section showing a population of RanBPM-expressing oocytes (blue) (G). Immunolabeling of germ cells using anti-MVH antibodies on the same section (H). Arrows indicate a sample of oocytes co-expressing RanBPM and MVH. Scale bars: 200 μm.