A) Both of the double stranded AAV7 viral vectors (scAAV7) contain one copy of a mutated inverted terminal repeat (ITR), the CMV enhancer and chicken β-actin promoter (CB promoter), cDNA of a single modifier gene tethered to a c-Myc/his tag, and the SV40 polyadenylation site. B) Western blot analysis of the proviral cassette expression in vitro probed against the reporter tag, c-Myc/his in 293T cells. The eGFP construct does not contain the c-Myc/his reporter tag and serves as the negative control for unspecific bands. rKiK3 is the original construct from which the c-Myc/his tag was obtained and shows a robust expression of the c-Myc/his sequence tethered to the KiK3 gene. For the Pcbp3 and Dnajb4 constructs, the proteins are expressed at the correct sizes of 36 kDa and 40 kDa respectively.