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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Mol Cell Neurosci. 2011 Mar 17;47(1):61–70. doi: 10.1016/j.mcn.2011.03.003

Figure 2. Plasmid design and proviral cassette expression in HEK 293T cells.

Figure 2

A) Both of the double stranded AAV7 viral vectors (scAAV7) contain one copy of a mutated inverted terminal repeat (ITR), the CMV enhancer and chicken β-actin promoter (CB promoter), cDNA of a single modifier gene tethered to a c-Myc/his tag, and the SV40 polyadenylation site. B) Western blot analysis of the proviral cassette expression in vitro probed against the reporter tag, c-Myc/his in 293T cells. The eGFP construct does not contain the c-Myc/his reporter tag and serves as the negative control for unspecific bands. rKiK3 is the original construct from which the c-Myc/his tag was obtained and shows a robust expression of the c-Myc/his sequence tethered to the KiK3 gene. For the Pcbp3 and Dnajb4 constructs, the proteins are expressed at the correct sizes of 36 kDa and 40 kDa respectively.