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. Author manuscript; available in PMC: 2011 May 24.
Published in final edited form as: Cereb Cortex. 2006 Apr 28;17(3):702–712. doi: 10.1093/cercor/bhk021

Figure 8.

Figure 8

Quantitative western blot analyses of HCN4 protein expression in developing hippocampus. (A) Top: Analysis of HCN4 protein levels in whole hippocampus reveals a progressive decrease of HCN4 protein expression between P2 and P90 (*denotes significance when compared with P2 and P11, †denotes significance when compared with P18; P < 0.05). Bottom: Representative western blot. Optical density (OD) of HCN4-immunoreactive bands was normalized to that of actin for each lane. (B) Top: Progression of HCN4 protein expression in isolated CA1 or DG + CA3 reflects the developmental profiles of the corresponding mRNA levels in these regions (compare with Fig. 7A). In CA1, HCN4 protein decreases ∼80% (corresponding mRNA decrease: 57%), whereas protein levels remain unchanged in DG + CA3 (*denotes significance between groups; t-test: P < 0.05). Bottom: Representative western blots illustrate significantly higher HCN4 protein levels in CA1 at P11. (C) Immunoreactive-HCN4 bands have an apparent molecular weight of ∼160 kDa (Notomi and Shigemoto 2004). Control experiments excluding the HCN4 antisera (-P) eliminate the immunoreactive bands.