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. Author manuscript; available in PMC: 2011 May 24.
Published in final edited form as: Brain Res Mol Brain Res. 2001 Nov 30;96(1-2):39–49. doi: 10.1016/s0169-328x(01)00265-0

Fig. 3.

Fig. 3

Abundance of CRH hnRNA in the PVN following cold stress. Densitometric analysis was performed on autoradiograms following in situ hybridization for CRH hnRNA in the PVN. Values are expressed as the means±S.E.M. of at least ten values from at least three brains per time-point. At both 6 (A) and 9 days (B) of age, there was greater than a two-fold increase in CRH hnRNA at 15 min after stress onset, and levels decreased at the 30 min time-point. One way ANOVA revealed a significant effect of treatment (F=8.53, P<0.0001). Post-hoc Bonferroni tests suggested that the 60 min time point values did not differ from the stress-free values for either group, although student’s t-test indicated significant (*) elevation in the 9-day-old group. Two way ANOVA demonstrated a strong effect of treatment (F=9.98, P<0.0001), and a trend towards an effect of age (F=2.81, P=0.096) that did not reach statistical significance.