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. 2011 Apr 26;50(21):4393–4395. doi: 10.1021/bi2004813

Figure 2.

Figure 2

Effect of benzbromarone on the stability of ΔF508 CFTR. (A) Pulse–chase assays of ΔF508 CFTR performed in the presence (+) or absence (−) of 0.05 mM benzbromarone (Benz). (B) Cell surface labeling performed on cells expressing ΔF508 CFTR in the presence (+Benz) or absence (−Benz) of 0.05 mM benzbromarone. Cells were first incubated for 24 h at 30 °C in the presence (+Benz) or absence (−Benz) of benzbromarone to promote maturation of the protein. Cell surface labeling was performed after the indicated times at 37 °C in 0.2 mg/mL cycloheximide. (C) Amounts of mature labeled CFTR at each time point (B) quantitated and expressed relative to time zero.