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(A) Cell lysates were prepared from Tpk analog-sensitive strain Y3527 (tpk1M164G tpk2M147G tpk3M165G) expressing the indicated HA-BCY1 deletions that were untreated or treated with 1 µM 1NM-PP1 for 90 min. Lysates were analyzed by SDS-PAGE and immunoblotting with anti-HA antibody. (B) Schematic diagram of Bcy1 deletion constructs indicating their ability to undergo phosphorylation when PKA is inhibited. Asterisk indicates non-specific band.
