Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Cell lysates were prepared from the following strains grown in 0.05% glucose to log phase: strain HS287-2C (bcy1Δ) carrying plasmids containing wild type HA-BCY1, HA-BCY1S77,79A or HA-BCY1S83,84A (lanes 1, 3 and 5) and strain HS293-10D (gpb1Δ gpb2Δ bcy1Δ) carrying plasmids containing wild type HA-BCY1, HA-BCY1S77,79A or HA-BCY1S83,84A (lanes 2, 4 and 6). Lysates were analyzed by SDS-PAGE and immunoblotting with anti-HA antibody.
