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. 2010 Nov 24;11(12):4782–4795. doi: 10.3390/ijms11124782

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© 2010 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Figure 1. — (A) Fraction 3 was not cytotoxic to keratinocytes, (□) ISO-1; (▪) Fraction 3; (▪) genistein. UVB irradiation-induced cell death decreased after treatment with ISO-1 and Fraction 3 by MTT assay. Results in (A) are expressed as percentage of control and the mean ± S.E. (n = 3). *P < 0.05 vs. control. (B) Effects of Fraction 3 and ISO-1 on UVB-induced ERK1/2, p38 and JNK phosphorylation. HaCaT cells were preincubated with Fraction 3 and ISO-1 for 24 h, followed by UVB irradiation. After incubation, cells were collected and lysates were analyzed by Western blot analysis, (F) Fraction 3; (I) ISO-1.