Table 1.

The factorial central composite design matrix for hydrolysis DH and yield rate: hydrolysis temperature (°C) (X₁), enzyme-to-substrate ratio (E/S) (units g⁻¹ protein) (X₂); substrate concentration (%) (X₃), reaction time (min) (X₄) were the four independent variables.

Trial No.	Independent Variables				DH (%)		Yield Rate (%)
	X1	X2	X3	X4	Actual Value	Predicted Value	Actual Value	Predicted Value
1	59.9	5852	6.8	174	21.29	21.04	52.54	52.07
2	59.9	5852	3.2	66	27.04	26.21	59.58	59.15
3	59.9	1898	6.8	66	15.07	15.10	41.47	40.08
4	59.9	1898	3.2	174	28.02	27.86	57.54	57.05
5	45.1	5852	6.8	66	24.21	24.24	46.68	47.02
6	45.1	5852	3.2	174	30.56	30.40	63.22	64.46
7	45.1	1898	6.8	174	19.5	20.20	49.32	49.60
8	45.1	1898	3.2	66	24.8	24.92	57.75	58.07
9	40	3875	5	120	23.84	23.36	53.89	52.514
10	65	3875	5	120	18.68	19.34	46.38	47.97
11	52.5	500	5	120	24.4	23.93	50.51	51.20
12	52.5	7200	5	120	29.08	29.74	59.21	58.73
13	52.5	3875	2	120	30.57	31.11	70.32	69.71
14	52.5	3875	8	120	19.37	19.00	48.2	48.71
15	52.5	3875	5	30	23.31	23.64	51.52	52.14
16	52.5	3875	5	210	27.58	27.44	60.47	60.06
17	52.5	3875	5	120	27.49	26.99	58.46	59.21
18	52.5	3875	5	120	27.69	26.98	56.4	59.21
19	52.5	3875	5	120	27.48	26.98	60	59.21
20	52.5	3875	5	120	26.97	26.98	61.03	59.21
21	52.5	3875	5	120	26.49	26.98	59.23	59.21
22	52.5	3875	5	120	26.49	26.98	60	59.21
23	52.5	3875	5	120	26.49	26.98	59.3	59.21