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Coded settings of the independent variables for DAM hydrolysis, according to central composite rotatable design: hydrolysis temperature (°C) (X1), enzyme-to-substrate ratio (E/S) (units g−1 protein) (X2); substrate concentration (%) (X3), reaction time (min) (X4) were the four independent variables.
| Coded Level |
Independent Variables |
|||
|---|---|---|---|---|
| X1 (°C) | X2 (units g−1 protein) | X3 (%) | X4 (min) | |
| 1.682(+γ) | 65 | 7200 | 8 | 210 |
| 1 | 59.9 | 5852 | 6.8 | 174 |
| 0 | 52.5 | 3875 | 5 | 120 |
| −1 | 45.1 | 1898 | 3.2 | 66 |
| −1.682(−γ) | 40 | 550 | 2 | 30 |
