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. 2011 Mar 15;10(6):989–998. doi: 10.4161/cc.10.6.15080

Figure 2.

Figure 2

Expression of dAven in Drosophila S2R+ cells. (A) Immunoblot of HA-tagged dAven expressed in S2R+ cells. Expression of dAven in S2R+ cells was achieved by transfection with a pMT/V5-His plasmid coding for HA-dAven followed by CuSO4 addition to the media to induce protein production. Extracts from these cells (transfected) and mock transfected controls (mock) were analyzed by PAGE followed by immunoblotting with anti-HA antibodies. (*) indicates a non-specific band present in both samples. (B) Immunolocalization of HA-tagged dAven transiently expressed in S2R+ cells. S2R+ cells were grown in coverslips and later transfected and induced as in (A). Subsequently, cells were fixed in paraformaldehyde, permeabilized with acetone and probed with anti-HA antibodies followed by anti-Rabbit-FITC conjugates. A representative immunofluorescence image is shown. Controls with anti-HA stain on cells transfected with an empty vector were completely devoid of signal at the exposure times analyzed (data not shown). Magnification 60X. Scale bar, 5 µm.