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Conditional knockout strategy via Cre-mediated DNA recombination. Cre-transgenic mouse is mated with mouse carrying floxed alleles. In the offspring mice, Cre transgene will be activated in targeted tissue. Cre catalyzes the recombination between two in cis loxP sites (red arrowheads), and excises the target gene from chromosome. loxP sequence(indicated by yellow circle) consists of an 8 bp nonpalindromic core flanked by two 13 bp inverted repeats. TSP: tissue-specific promoter.
