Figure 5.

Density of unselected mutations and purine/pyrimidine bias in subtelomeric and internal regions of yeast chromosomes revealed by whole genome sequencing. The letter “m” precedes the number of specific mutant strains. In order to obtain parameters for unselected mutations, all changes in the 25 kb left subtelomeric region of chromosome V containing the LYS2 mutation reporter were excluded from calculations. (A) Density of mutations calculated for the total of base substitutions, indels and complex changes. (B) Purine/pyrimidine bias of simple base substitutions. Mutations were categorized based on nucleotide changes in the 5′→3′ (top) strand throughout the chromosome reference sequence.