Figure 1.

Shox2 expression in the developing DRG. A, In situ hybridization experiments show that at E10.5 and E11.5 Shox2 is expressed throughout the entire DRG; at E14.5, Shox2 expression begins to downregulate in subsets of DRG neurons; and by E18.5 and P0, Shox2 is only present in a small number of DRG neurons. B, Two-color in situ hybridization with probes for Shox2 (red) and different receptors (TrkA, TrkB, TrkC, and Ret) (green). Arrows indicate cell bodies in the ganglia showing colocalization of Shox2 with either TrkB or Ret. Note that not all TrkB- or Ret-positive cell bodies have Shox2 expression at P0. Scale bar, 100 μm.