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. Author manuscript; available in PMC: 2011 May 24.
Published in final edited form as: Cancer Res. 2008 Dec 1;68(23):9663–9670. doi: 10.1158/0008-5472.CAN-08-2229

Fig. 2. IR induces cytoplasmic accumulation of ATF2 and an increase in nuclear pCREB in LNCaP cells.

Fig. 2

(A) LNCaP Prostate cancer cells cultured in 12-well plates were treated with DMSO or LMB (40 ng/ml) overnight or irradiated (2 Gy/day) for 5 days followed by treatments with DMSO or LMB overnight. Subcellular localization of ATF2 was determined by immunostaining with anti-ATF2 antibody, and DNA in the nucleus was stained with DAPI. (B) and (C) Non-irradiated (IR-) or irradiated LNCaP cells (2 Gy x 5) (IR+) were harvested, and cytosolic, nuclear and total cellular extracts were prepared. Approximately 20 μg of total cellular extracts (T) and an equal portion of cytosolic (C) and nuclear (N) extracts were used for immunoblotting of ATF2, pCREB and CREB.