Fig. 5.

Hsp90_Ec interacts with DnaK. (A) A bacterial two-hybrid assay was used to detect interactions between Hsp90_Ec and DnaK in vivo as described in Methods. Interactions were detected by measuring β-galactosidase activity in E. coli extracts. Data from three replicates are presented as mean ± SEM. (B) In vitro interaction between DnaK and Hsp90_Ec was monitored by measuring retention of [³H]DnaK on filters with a 100-kDa exclusion limit in the presence of Hsp90_Ec, BSA, or S. cerevisiae Hsp82 as described in Methods. Data from at least six replicates are presented as mean ± SEM.