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Delivery of Dig-payloads. (A) MCF-7 were incubated in the absence (cells only) or presence of Dig-bispecifics targeting LeY (LeY-Dig) or CD22 (CD22-Dig) and analyzed by FACS in absence or presence of Dig-Cy5. (B) Raji incubated in absence or presence of Dig-Cy5 or Dig-Cy5 complexed CD22-Dig. (C) MCF-7 incubated with Dig-eGFP or Dig-eGFP complexed with LeY-Dig analyzed in the FITC channel. Details of the detection procedures are provided in SI Text.
