Fig. 6.

Targeting and internalization of payloads. (A) MCF-7 incubated on ice for 2 h with 40 nM LeY-Dig or 40 nM CD22-Dig complexed with Dig-eGFP, or with uncomplexed Dig-eGFP. Cells were fixed (Upper) or incubated for another 3 h at 37 °C (Lower). CY3-labeled goat anti-human IgG was used for visualization by confocal laser scanning microscopy. (B) MCF-7 incubated as in A with Dig-Cy5 as payload. (C) MCF-7 incubated as in A with Dig-Dox as payload. A detailed description of the procedures is provided in SI Text.