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Payload targeting in vivo. (A) IGF1R-Dig bispecifics charged with Dig-Cy5 were applied to H322M xenografts. IGF1R-Dig carries Dig-Cy5 to the xenograft, Dig-Cy5 without antibody appears in liver (circles) and becomes eliminated (Fig. S7). (B) Her2-Dig bispecifics specifically targets Dig-Dox to Calu3 xenografts. (C) Preapplication of bispecifics followed by Dig-Cy5 leads to specific capture of Dig-Cy5 at the tumor.
