Fig. 3.

Unrepaired clustered DNA lesions result in high levels of gross-chromosomal aberrations in Fe ion-irradiated cells. (A) The total number of gross chromosomal aberrations per mitotic cell. (B) The number of different types of chromosomal aberrations per mitotic cell after 1 Gy of γ-ray, O, Si, and Fe irradiation. Exponentially growing HT1080 cells were irradiated with 1 Gy of radiation, and 16 h later the chromosome preparations were made by accumulating mitotic cells in the presence of 0.1 mg/mL colcemid for 8 h. For each radiation type, more than 100 metaphase spreads were counted. Each data point in the graph is the average of three independent experiments. Error bars represent SD.