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Fluorescent detection of O-GlcNAc-modified proteins. (A) Fluorescent-tags Alk-Rho and Az-Rho for CuAAC-dependent detection of GlcNAz and GlcNAlk (B) HEK293 cells were treated with the indicated concentrations of Ac4GlcNAz or Ac4GlcNAlk for 16 h and analyzed by in-gel fluorescence scanning. Coomassie blue staining demonstrated equal protein loading. (C) Higher contrast of lanes corresponding to 0 μM in B to allow for comparison of background levels.
