Fig. 2.

H3K4 global methylation is differentially affected by loss of SET-2 and ASH-2. The levels of histone H3K4me3 (A, C, and E) and H3K4me2 (B and D) were assayed using quantitative Western blots and normalized to levels of histone H3 and actin (Materials and Methods). (A–D) Total cell extracts were derived from synchronized populations of wild-type and mutant strains at the indicated developmental stages. (E) Levels of H3K4me3 were compared between wild type, set-2(bn129), set-2(bn129)glp-1, and glp-1 young adults. Bar graphs depict the combined results from three independent biological replicates; error bars indicate SD.