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. 2011 May 2;108(20):8408–8413. doi: 10.1073/pnas.1019209108

Fig. 3.

Fig. 3.

Accumulation of ubiquitinated proteins and apoptosis are induced by loss of Nrf1 in neurons. (A) Nrf1 expression in Nrf1flox/flox;Cre-ERT2 neuronal cultures treated with DMSO or 4HT. Nrf1 mRNA levels were measured by quantitative RT-PCR. Data were normalized to the 18s rRNA; means ± SEM, n = 3. *P < 0.05. Inset: Western blot analysis of Nrf1 in DMSO and 4HT treated Nrf1flox/flox;Cre-ERT2 neuronal cells. (B) Quantitation of ubiquitin immunostaining in Nrf1flox/flox;Cre-ERT2 neuronal cultures at various days after treatment with DMSO or 4HT. (C) Quantitation of TUNEL labeling in Nrf1flox/flox;Cre-ERT2 neuronal cultures at various days after treatment with DMSO or 4HT.