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. 2011 May 2;108(20):8408–8413. doi: 10.1073/pnas.1019209108

Fig. 7.

Fig. 7.

Nrf1 regulates PsmB6 gene. (A) Transactivation of the mouse PsmB6 promoter by Nrf1. PsmB6 luciferase reporter was transfected along with vector, Nrf1, or Nrf2 cDNA into 293 cells. Activities represent the mean of at least three independent experiments ± SEM. *P < 0.05. (B) Activity of the PsmB6 luciferase reporter in wild-type, Nrf1−/−, Nrf2−/−, or Nrf1−/− fibroblasts expressing Nrf1 cDNA, Nrf2 cDNA, or Nrf1 cDNA containing a deletion in the bZIP domain. Results represent the mean of at least three independent experiments ± SEM. *P < 0.05. (C) Chromatin immunoprecipitation of PsmB6 promoter in vivo. Graph represents real-time PCR amplification of chromatin template precipitated with either anti-Nrf1 or rabbit preimmune IgG. Primers were targeted to various regions spaced 500 bp apart along the PsmB6 promoter. NQO1 and LDH promoters were used as positive and negative controls, respectively. Nonimmunoprecipitated chromatin (1%) was used as an input control. The open box indicates the location of a consensus-binding site for Nrf1 in the PsmB6 promoter. (D) Activity of wild-type PsmB6 promoter and mutant promoter containing a deletion of the −38 ARE site. Reporter constructs were transfected along with vector or Nrf1 cDNA into MEF cells. Activities represent the mean of three independent experiments ± SEM. *P < 0.05.