Fig. 3.
Effect of salt on colony growth in Physcomitrella wild-type and med25a strains. (A) Representative pictures of colonies from each of the three med25a knockout strains and the wild-type control after 21 d in normal light intensity (30 μmol/m2 s). The top row shows growth on BCD with 0.15 M NaCl. The middle row shows BCD with 0.30 M mannitol as osmotic control, and the bottom row shows just BCD. (B) Average colony diameter of wild-type and med25a strains under different conditions. The mean values from four colonies (WT) or 12 colonies (med25a) ± SEM are shown. The significances of the observed differences were tested using a two-tailed two-sampled t test assuming unequal variances. The asterisk denotes a difference that is significant at p = 0.0014.