Fig. 5.

Overexpression of TFC6 increases TF_IIIC association at multiple genomic loci. Strain DDY4381 (TFC1-3×FLAG) was transformed with either empty vector or pDD1234 (ADH1 promoter-TFC6) to overexpress Tfc6p. Binding of TF_IIIC was assessed at several B-box sites by ChIP using anti-FLAG antibody; each B-box site showed increased enrichment when Tfc6p was overexpressed. As in Fig. 3, determinations were performed in triplicate and normalized to the GAL locus signal; one pair of lanes is shown for each locus.