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. 2011 Apr 27;108(20):8485–8490. doi: 10.1073/pnas.1103029108

Fig. 2.

Fig. 2.

RO5166017 inhibits the firing rate of DA and 5-HT neurons but not that of NA neurons. (A, C, and E) Representative recordings from brain slices of WT and Taar1−/− mice. (Scale bar: VTA, 20 mV/s; DRN and LC, 30 mV/s.) (B, D, and F) Quantification bar graphs (mean ± SEM; n = 5 neurons, recorded from three animals per condition). The firing frequency was assessed before (Control) and during application of RO5166017 (500 nM; RO) alone and in combination with EPPTB (10 nM). In WT mice, RO5166017 decreased firing of DA neurons in the VTA (A and B) and 5-HT neurons in the DRN (C and D), whereas application of EPPTB increased the firing rate above control levels. In brain slices of Taar1−/− mice, the spontaneous firing frequencies of the DA and 5-HT neurons were increased compared with WT, and they were not affected by RO5166017. In the LC (E and F), the spontaneous firing rates of NA neurons in WT and Taar1−/− mice were not significantly different, and RO5166017 had no effect in WT mice. ***P < 0.001 vs. the other two conditions.