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PI metabolism by cultured colon cancer cells. PI and its metabolites were extracted from the PI-treated HCT-15 cell lysates and analyzed using HPLC and LC/MS/MS as described under Materials and Methods. Top, MS spectrum of PI obtained from LC separation. Inset, HPLC chromatogram of extract from HCT-15 cells treated with PI; only PI was detected by UV detector. Bottom, MS/MS spectrum of fragment ions of the PI.
