Figure 3.

Cell-binding ability of B18 and its N- and C-terminal fragments. A) Schematic representation of the mature recombinant B18-tagged protein comprising 3 Ig domains, and the 2 B18-N and B18-C truncated versions of B18 generated. Dark boxes illustrate the location of the putative GAG-binding regions identified. B) Flow cytometric analysis (as described in Fig. 2) of HeLa cells incubated with increasing volumes of supernatants (0–40 μl) containing the corresponding tagged proteins. One representative experiment of 2 is shown. Legend: volumes corresponding to traces. C) Western blot with anti-V5 antibody monitoring the protein amounts used in the experiment. Molecular mass markers (kDa) are at left.