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. 2011 Jun;25(6):2082–2091. doi: 10.1096/fj.10-176891

Figure 5.

Figure 5.

ATP failed to activate ERK in apoE−/− microglia. A) RT-PCR analysis of mRNAs from wild-type (wt) and apoE−/− microglia shows expression of purinergic receptors. B) P2X4 and P2Y12 receptor expression was confirmed using Western blot analysis of whole-cell lysates isolated from untreated cultured microglia. C) Western blot analysis shows time course of ERK activation (pERK) in whole-cell lysates prepared from cultured murine microglia stimulated with 300 μM ATP over 30 min. Quantification revealed failure of ATP to induce ERK activation in apoE−/− microglia over 30 min. Western blots are representative of ≥3 independent experiments. Data are expressed as percentage of ERK over time 0 control. ***P < 0.001 vs. corresponding basal; ANOVA withy Dunnett's multiple comparison test.