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. 2011 Jun;25(6):1844–1855. doi: 10.1096/fj.10-169797

Figure 2.

Figure 2.

AGS3 colocalizes with proliferation marker Ki-67. A–L) Representative images of Ki-67 in the cortex (A–D) and outer medulla (E–L) from sham-operated (A, E, I) and 48-h bilateral IRI-treated rats (B–D, F–H, J–L). Images are from the renal outer medulla in two different rats. Arrows in panels C, G, and K indicate areas magnified in panels D, H, and L, respectively. Ki-67 (brown) and AGS3 (red) were detected by DAB and Vulcan fast red staining, respectively. Arrowhead in panel C shows an AGS3-deficient tubule in the absence of Ki-67 unlike the adjacent Ki-67+ tubule (arrow) with detectable levels of AGS3 protein expression. All sections were counterstained with hematoxylin. M) Quantitation of Ki-67+ epithelial cells in the renal cortex (Ctx) and outer medulla (OM) as a percentage of total tubular epithelial cells. N) Quantitation of AGS3+ epithelial cells in OM as a percentage of total tubular epithelial cells. O) Quantitation of Ki-67 and AGS3 double-positive epithelial cells in OM as a percentage of the total tubular epithelial cells. n = 3–5 samples/group, as indicated on bars. *P < 0.05, **P < 0.001 vs. sham-operation group.