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. 2011 Feb 7;192(3):513–524. doi: 10.1083/jcb.201010003

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© 2011 Poukkula et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 6. — Effects of perturbing both guidance receptors. (A) Net movement of border cell clusters. Genotypes (for all panels): slboGal4, UAS-10×GFP/+ (control [con]) or UAS-DN-EGFR/UAS-DN-PVR;slboGal4, UAS-10×GFP/+ (D-DN) or hsFLP/+;UAS-EgfrRNAi/+;UAS-PvrRNAi/AFG, UAS-10×GFP (D-RNAi) and hsFLP/+;AFG, UAS-10×GFP/+ (con^AFG; n = 37, 38, 18, and 22, respectively). SEM is indicated. AFG (actin-flipout-Gal4) was activated 2 d before imaging to give expression in all somatic cells, including border cells. Only migration at stage 9, up to 50% of path (early), was analyzed. The controls looked similar in all regards. (B) Apparent single-cell speed (tracked nuclei). n = 12–17. (C) Extensions per frame (n > 1,300); all differences are significant with P < 0.001, except the side versus back of D-RNAi (P < 0.05). (D) Mean lifetime of extensions (P(t)); there were significant differences (P < 0.02) for front extensions in different genotypes and compared with side and back extensions. (E) Direction of new extensions in percentages; indicated below is the total number of extensions per hour (n > 200). (F) Mean extension areas in micrometers squared based on all snapshots of extensions; all differences are significant (P < 0.01), except for side extensions and control versus D-RNAi. (D and F) n = 150–266.