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. 2011 Feb 7;192(3):447–462. doi: 10.1083/jcb.201008177

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© 2011 Claypool et al.

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Figure 4. — The BTHS mutant tafazzins are degraded by the i-AAA protease. (A and B) Steady-state expression was determined from whole-cell extracts derived from the indicated strains by immunoblotting for Taz1p, Yme1p, cytochrome c peroxidase (CCPO), and the loading control, porin. n = 3. (C) Increased half-life of the BTHS mutants in the absence of Yme1p. Whole-cell extracts were harvested after incubation with cycloheximide (CHX) for the indicated times, and the Taz1p remaining was determined by immunoblotting. Yme1p and porin are mitochondrial controls, and hexokinase (Hxk1p) is a cytosolic control. The four BTHS mutants being characterized in the present study are shown in red. Relative molecular masses are shown on the left.