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. 2011 Feb 7;192(3):481–495. doi: 10.1083/jcb.201004016

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© 2011 Sen et al.

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Figure 2. — Heartless localizes to the postsynaptic region of the Drosophila larval NMJ. All panels show wild-type NMJs derived from larval muscle 4. (A, D, G, and J) Htl (green) expression in the NMJ boutons. (B) Dlg (red) marks the postsynaptic region of the NMJ. (C) Htl (green) and Dlg (red) expression coincide at the larval NMJ. (F) Htl (green) and the presynaptic nc82 (red) expression do not overlap at the NMJ boutons. (I) Mutually exclusive expression of Htl (green) and presynaptic marker Csp (red) at the larval NMJ. (L) Htl (green) expression does not colocalize with presynaptic HRP staining (red). (M and N) qPCR from mRNA derived from tissues extracted from third instar larvae reveals the expression of htl and its ligands, ths and pyr, in the brain (M) and muscle (N). Bar, 5 µm.