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. 2011 Feb 7;192(3):481–495. doi: 10.1083/jcb.201004016

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© 2011 Sen et al.

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Figure 3. — heartless signaling regulates NMJ morphology. (A) Wild-type NMJ derived from larval muscle 4. (B) Drastic reduction in NMJ size in how24BGAL4 animals driving expression of a transgenic construct carrying a dominant-negative heartless (UAS-htl^DN). The how24BGAL4 driver expresses GAL4 predominantly in the muscles. (C) Reduction in NMJ size by overexpression of Sprouty. (D) Expansion of the NMJ in animals overexpressing Stumps by how24BGAL4. (E) Neuronal overexpression of htl RNAi by elavGAL4 has no effect on NMJ size or morphology. (F) Neuronal overexpression of stumps RNAi has no effect on NMJ size and/or morphology. (G) Quantitation of bouton number/muscle in animals of the indicated genotypes, normalized to muscle surface area (MSA) as a percentage of wild type (WT). how24BGAL4/+ animals are used as controls. The ANOVA multiple comparison test was used for statistical analysis of the bouton number/muscle. P ≤ 0.05. Bar, 50 µm. n = 40.