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. 2011 Feb 7;192(3):481–495. doi: 10.1083/jcb.201004016

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© 2011 Sen et al.

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Figure 6. — Overexpression of Htl rescues NMJ defects caused by reduced Smn. how24BGAL4 expresses GAL4 predominantly in the mesoderm (muscles). (A) Wild type NMJ derived from larval muscle 4. (B) Reduction in NMJ size in how24BGAL4 UAS-Smn-RNAi^N4/+ animals. (C) The how24BGAL4 UAS-Smn-RNAi^N4/+ and (D) how24BGAL4 UAS-Smn-RNAi^C24/+ NMJ size defects are rescued by overexpression of Htl (UAS-htl^wt). (E) how24BGAL4/UAS-stumps individuals show an expansion in NMJ size. (F) The how24BGAL4 UAS-Smn-RNAi^C24/+ NMJ size defects are rescued by overexpression of Stumps (UAS-stumps). (G) Quantitation of bouton number/muscle in animals of different genotypes, normalized per muscle surface area (MSA) as a percentage of wild type (WT). how24BGAL4; UASGFP is used as control. The ANOVA multiple comparison test was used for statistical analysis of the bouton number/muscle. P ≤ 0.05. Bar, 50 µm. n = 40. All preparations were stained with anti-HRP (red) and anti-Dlg (green). The muscle nucleus was labeled using DAPI.