Table 1.
Primers and annealing temperatures for PCR amplification of each gene studied.
| SYBR® Green RT-PCR: | |||
|---|---|---|---|
| Primers | Sense primer | Antisense primer | Annealing Temperature |
| ACC | 5'-GGACCACTGCATGGAATGTTAA-3' | 5'-TGAGTGACTGCCGAAACATCTC-3' | 60.6°C |
| FAS | 5'-AGC CCC TCA AGT GCA CAG TG-3' | 5'-TGCCAATGTGTTTTCCCTGA-3' | 60.0°C |
| SREBP-1c | 5'-GCGGACGCAGTCTGGG-3' | 5'-ATGAGCTGGAGCATGTCTTCAAA-3' | 67.3°C |
| ATGL | 5'-CACTTTAGCTCCAAGGATGA-3' | 5'-TGGTTCAGTAGGCCATTCCT-3' | 62.0°C |
| Perilipin | 5'-AGAGGAGACAGATGAGGAGGAAG -3' | 5'-AGATGGTGTTCTGCAGAGTCTTC -3' | 63.9°C |
| LPL | 5'- CAGCTGGGCCTAACTTTGAG -3' | 5'- CCTCTCTGCAATCACACGAA -3' | 61.5°C |
| PPARγ | 5'-ATTCTGGCCCACCAACTTCGG -3' | 5'-TGGAAGCCTGATGCTTTATCCCCA -3' | 64.5°C |
| β-Actin | 5'-ACGAGGCCCAGAGCAAGAG-3' | 5'-GGTGTGGTGCCAGATCTTCTC-3' | 60.0°C |
ACC, acetyl-CoA carboxylase; FAS, fatty acid synthase; SREBP-1c, sterol-regulatory element binding protein-1c; ATGL, adipose triglyceride lipase; LPL, lipoprotein lipase; PPAR, peroxisome proliferator-activated receptor.