Figure 3.

Analysis of Apoptosis using DiLC and PI. Comparison of two different methods for measurement of apoptosis, DilC and Annexin V in human ESC (pluripotent cells) or Jurkat cells (Differentiated cells). Flow cytometry histogram of hESC assessed for apoptosis levels using DiLC, which measures the mitochondrial membrane potential. Note that the control ESC treated with CCCP (with DiLC at the same time) to show the shift in signal, demonstrating that the assay is working. Cyclosporin A was used in Annexin V assays to induce apoptosis (50 uM for 20 hours).