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MoFlo Instrument Settings the Flow Cytometry machine for detection of GFP, EdU and DAPI signals.
| Laser | Excitation wavelength & power | Band Pass Filters used for collection of emitted fluorescence (wavelength/power) |
|---|---|---|
| Blue | 488 nm at 30 mWatts | Green emission - 530/30 (from anti-GFP/Alexa Fluor 488) |
| Ultra violet | 351 nm at 20 mWatts | Blue emission - 450/65 (from DAPI) |
| Red | 633 nm at 35 mWatts | Red emission - 670/20 (from Alexa Fluor 647 azide linked to EdU) |
